|
KMID : 0361020000430050471
|
|
Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 5 p.471 ~ p.475
|
|
|
The Effects of All-trans-retinoic Acid on the Proliferation of Cultured Keratinocytes from External Ear and Cholesteatoma
|
|
Bang Kin-Hyun
Yoon Tae-Hyun
|
|
|
Abstract
|
|
|
Background and Objectives : Cholesteatoma is a disease of the temporal bone, affecting
the squamous epithelium and invading into the middle ear cavity. Retinoic acid (RA) is
known to inhibit or accelerate the proliferation of normal keratinocytes depending on its
concentration. This study was aimed at investigating the effects of different
concentrations of RA on the proliferation of cultured keratinocytes from the external ear
and cholesteatoma and whether the effect is different according to the origin of tissue.
Materials and Methods : Patients undergoing tympanomastoid surgery for cholesteatoma
were selected, and cholesteatoma matrix and skin were obtained from their middle ears
and external ears, respectively. After culturing keratinocytes from the external ear
cholesteatoma, cells were cultured for 7 days in the medium 154(Cascade, USA), and
10-6 M RA or 10-7 M RA was added twice every three
days. The control group was cultured without the addition of RA. The absorbance was
estimated at 490 using CellTiter 96TM AQucous
Non-Radioactive Cell Proliferation Assay, nm and the survival fraction was calculated
and compared with the control group.
Results : Proliferation of keratinocytes from the external ear and cholesteatoma was
inhibited when the concentration of RA was 10-6 M and accelerated when
the concentration of RA was 10 M. The accelerated effect of retinoic acid
on the proliferation of keratinocyte seems to be less prominent in the cholesteatoma
tissue than in the skin of normal ear canal.
Conclusion : Retinoic acid can inhibit or accelerate the proliferation of cholesteatoma
keratinocytes dependining on its concentration.
|
|
|
KEYWORD
|
|
|
Retinoic acid, Keratinocyte, Cell proliferation, Cholesteatoma
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|